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Unleash Your Sequencing Speed

As one of the sequencers with the fastest speed among mid-low throughput in the world, DNBSEQ-G99⁽¹⁾is developed based on MGI's core DNBSEQ™ sequencing technology, enabled by innovations in biochemistry, optics, fluidics, temperature control, and other core systems. DNBSEQ-G99 is especially applicable for multiple sequencing methods, it only needs 12 hours to finish PE150 sequencing with Q40 data. Different types of flow cells can be selected for sequencing according to the sample quantity.

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Features

8 Gb~192 Gb per run

Only 12 hrs for PE150 (from loading to FASTQ)

Supports multiple read lengths, including PE50, SE100, PE150, PE300, SE400

Applicable to multiple sequencing methods

Performance Parameter

Maximum number of Flow Cells	Lanes/Flow Cell	Flow Cell Type	Reads/Flow Cell*	Supported Read Lengths	Data Output	Q30***	Q40***	Run Time***
2	1	FCS	40 M	App-D PE150	12 Gb~24 Gb	>90%	>85%	~11 h
		FCL	80 M	SE100/PE50	8 Gb~16 Gb	>90%	>85%	~5 h
				PE150	24 Gb~48 Gb	>90%	>85%	~12 h
				App-D SE100	8 Gb~16 Gb	>90%	>85%	~5 h
				App-D PE150	24 Gb~48 Gb	>85%	>85%	~12 h
				App-D PE300	48 Gb~96 Gb	>85%	>85%	~30 h
				SE400	32 Gb~64 Gb	>75%	>70%	~20 h
		FCU	160 M	App-D SE100	16 Gb~32 Gb	>90%	>85%	~7 h
				App-D PE150	48 Gb~96 Gb	>85%	>85%	~16 h
				App-D PE300	96 Gb~192 Gb	>85%	>80%	~35 h
* Effective reads is determined using a standard library. Actual output may vary depending on sample type and library preparation method. The percentage of bases above Q30 and run time is the average of an internal standard library over the entire run. The actual performance is affected by factors such as sample type, library quality, and insert fragment length. Only StandardMPS 2.0(SM 2.0) reagents support the generation of Q40 data. * The sequencing time is the statistical duration for single flow cell sequencing and dual flow cell simultaneous sequencing. ★ The instrument is equipped with SE50 and PE100 sequencing modes, and the existing reagent kits support SE50 and PE100 read length sequencing. Note: APP-D has built-in Illumina’s Truseq, Nextera adapters, and MGI adapter, which supporting mixed testing of Illumina’s Truseq, Nextera adapters, and MGI adapter libraries.

Method	Application	Recommended read length	Recommended data size	FCS	FCL	FCU
Method	Application	Recommended read length	Recommended data size	Recommended sample /RUN
Target capture/multiplex PCR	Onco panel	PE100/PE150	Small panel: ~1 Gb/sample	12/FC，24/RUN	24/FC，48/RUN	48/FC，96/RUN
	Genetic disease diagnosis small panel (Thalassemia, deafness, etc.)	PE150	Deafness: ~5 Gb/sample	2/FC，4/RUN	4/FC，8/RUN	9/FC，18/RUN
		PE150	Thalassemia: ~0.2 M reads/sample	200/FC，400/RUN	400/FC, 800/RUN	800/FC, 1600/RUN
	ATOPlex panel (Respiratory, COVID-19, etc.)	PE100/PE150	Respiratory panel: 5M reads/sample COVID-19 panel: 5M reads/sample	8/FC，16/RUN	16/FC，32/RUN	32/FC，64/RUN
	WES	PE150	15 Gb/sample	/	1-2/FC，2-4/RUN	3/FC，6/RUN
Methylation	Onco targeted methylation panel	PE150	~5 Gb/sample	2/FC，4/RUN	4/FC，8/RUN	9/FC，18/RUN
Small genome sequencing	Metagenomics for pathogen detection	SE50/SE100	Meta：20 M reads/sample	/	4/FC，8/RUN	8/FC，16/RUN
	Microbial WGS sequencing	PE100/PE150	Single bacterium: ~1 Gb/sample	12/FC，24/RUN	24/FC，48/RUN	48/FC，96/RUN
	16S sequencing	PE300	～0.1 M reads/sample	/	640/FC，1280/RUN	640/FC，1280/RUN
Low-pass WGS sequencing	NIPT	SE50	NIPT/PGS：~10 M reads/sample	/	8/FC，16/RUN	16/FC，32/RUN
Low-pass WGS sequencing	PGS	SE50	NIPT/PGS：~10 M reads/sample	/	8/FC，16/RUN	16/FC，32/RUN
Transcriptome sequencing	RNA-Seq	SE50/PE150	Expression profiling: ~25 M/sample	/	3/FC，6/RUN	6/FC，12/RUN
Transcriptome sequencing	RNA-Seq	SE50/PE150	Transcriptome: ~6 Gb/sample	/	4/FC，8/RUN	8/FC，16/RUN
Forensics	DNA signature identification	SE400	~0.8 M reads/sample	/	96/FC，192/RUN	/

Ordering Information

Cat. No	Product Name
900-000611-00	DNBSEQ-G99RS
900-000608-00	DNBSEQ-G99ARS
940-002649-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 App-D FCS PE150)
940-001268-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 FCL SE100/PE50)
940-001269-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 FCL PE150)
940-001267-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 App-D FCL SE100)
940-001274-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 App-D FCL PE150)
940-001716-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 App-D FCL PE300)
940-001757-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 FCL SE400)
940-002776-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 App-D FCU SE100)
940-002775-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 App-D FCU PE150)
940-002773-00	DNBSEQ-G99RS High-throughput Sequencing Set (G99 App-D FCU PE300)
940-000624-00	DNBSEQ-G99RS Cleaning Reagent Kit (G99 SM FCL)
UPS	Selected as needed

System Parameter

Product Model
DNBSEQ-G99 (Without Bioinformatics Module)

DNBSEQ-G99A (With Bioinformatics Module)

DNBSEQ-G99RS (Without Bioinformatics Module)⁽¹⁾

DNBSEQ-G99ARS (With Bioinformatics Module)⁽¹⁾

^{(1) For research use only. Not for use in diagnostic procedures.}